Pickle and his colleague Charles Hedgehog were doing research here when they were abducted by Dr. Eggman's forces under E-106 Eta. Chuck's assistant Ben Muttski subsequently contacted the Knothole Freedom Fighters with news of this event. (StH: #257)
- Spagonia University first appeared in the game Sonic Unleashed.
- Before appearing in the comic, the University was first mentioned in the Sonic Super Digests short-lived "Professor Pickles Nature Notebook" feature. This was when the comic was still in its original Pre-Super Genesis Wave Timeline, implying that it existed in that timeline, but this has yet to he confirmed.